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IN VITRO
MORPHOGENIC PERFORMANCE OF GINGER (Zingiber officinale) AS INFLUENCED BY MEDIA
VARIATIONS
ABSTRACT
Ginger (Zingiber officinale Rose) is a
perennial herb that grows from underground rhizomes. The demand for fresh and
dry ginger and its essential oil in the world market is high. Ginger is
propagated vegetatively using underground rhizomes. Most farmers use planting
materials saved from previous harvest. These materials could have been sold for
cash. Contingent on this, many farmers are reluctant to use healthy succulent
rhizomes for planting. These are rather sold thereby resulting in acute
shortage of planting materials.
Tissue culture technique can be applied to
mass-produce seedlings for distribution to ginger farmers. This however, is not
cost effective now due to lack of the necessary materials such as agar required
for tissue culture. It is on the basis of these considerations that the present
research was set up to develop a locally available and cheap protocol that is
reproducible in ginger plant tissue culture work with the following objectives:
To determine the best pre-initiation treatment
for in-vitro ginger multiplication; To determine the effect of plant growth
hormones – auxin and cytokinin on in-vitro propagation of ginger at the initiation
stage using agar as a gelling agent; To compare agar gelled medium with cassava
gelled medium in in-vitro propagation of ginger at the initiation stage. The
research was carried out in the tissue culture research laboratory of the
National Root Crop Research Institute (NRCRI), Umudike Station, Umuahia, Abia
State. The explants were collected from ginger germplasm of the Institute. The
result shows that Sodium Hypochlorite (NaOCl) as 30% bleach and
TABLE
OF CONTENT:
CHAPTER ONE
INTRODUCTION
1.1
Background of the Study
1.2
Statement of the Research Problem
1.3
Objectives of the Study
1.4
Significance of the Study
1.5
Research Questions
1.6
Research Hypothesis
1.7
Conceptual and Operational Definition
1.8
Assumptions
1.9
Limitations of the Study
CHAPTER TWO
LITERATURE REVIEW
2.1
Sources of Literature
2.2
The Review
2.3
Summary of Literature Review
CHAPTER THREE
RESEARCH METHODOLOGY
3.1
Research Method
3.2
Research Design
3.3
Research Sample
3.4
Measuring Instrument
3.5
Data Collection
3.6
Data Analysis
3.7
Expected Result
CHAPTER FOUR
DATA ANALYSIS AND RESULTS
4.1
Data Analysis
4.2
Results
4.3
Discussion
CHAPTER FIVE
SUMMARY AND RECOMMENDATIONS
5.1
Summary
5.2
Recommendations for Further Study
References
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